Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda

• 作者: JlN Ming-hui TAO Jia-hui Ll Qi CHENG Ying SUN Xiao-xu WU Kong-ming XlAO Yu-tao
• 来源期刊: 农业科学学报（英文）
• 年，卷（期）: 2021,（3）
• 所属期刊栏目: Section 4:Integrated pest management
• 页码范围: 815-820
• 页数: 6页
• 语种: 英文
• DOI: 10.1016/S2095-3119(19)62772-3

摘要

ATP-binding cassette transporter C2 (ABCC2) is known to be a receptor for Bacillus thuringiensis (Bt) toxins in several lepidopteran insects.Mutations in the ABCC2 gene have been genetically linked to field-evolved resistance to the Cry1F toxin from Bt in Spodoptera frugiperda.Here we generated a SfABCC2 knockout strain of S.frugiperda using the CRISPR/Cas9 system to provide further functional evidence of the role of this gene in susceptibility and resistance to Cry1F.Results from bioassays showed that the SfABCC2 knockout S.frugiperda strain displayed 118-fold resistance to Cry1F compared with the parental DH19 strain,but no resistance to Vip3A toxin from Bt.These results provide the first reverse genetic evidence for SfABCC2 as a functional receptor for Cry1F.