ERF1 delays flowering through direct inhibition of FLOWERING LOCUS T expression in Arabidopsis

• 作者: Yanli Chen Liping Zhang Haiyan Zhang Ligang Chen Diqiu Yu
• 来源期刊: 植物学报（英文版）
• 年，卷（期）: 2021,（10）
• 所属期刊栏目: Molecular Physiology
• 页码范围: 1712-1723
• 页数: 12页
• 语种: 英文

摘要

ETHYLENE RESPONSE FACTOR1 (ERF1) is a key component in ethylene signaling, playing crucial roles in both biotic and abiotic stress responses. Here, we demonstrate that ERF1 also has an im-portant role during floral initiation in Arabidopsis thaliana. Knockdown or knockout of ERF1 accel-erated floral initiation, whereas overexpression of ERF1 dramatically delayed floral transition. These contrasting phenotypes were correlated with opposite transcript levels of FLOWERING LOCUS T (FT). Chromatin immunoprecipitation (ChIP) as-says revealed that ERF1 associates with genomic regions of the FT gene to repress its transcription. ft-10/ERF1RNAi plants showed a similar flowering phenotype to the ft-10 mutant, whereas the flow-ering of FTox/ERF1ox mimicked that of FTox plants, suggesting that ERF1 acts upstream of FT during floral initiation. Similarly, altered floral transition in ethylene-related mutants was also correlated with FT expression. Further analysis suggested that ERF1 also participates in delay in flowering-time control mediated by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid. Thus, ERF1 may act as a neg-ative modulator of flowering-time control by re-pressing FT transcription in Arabidopsis.