Cigarette smoke extract-induced 16HBE-derived exosomal miR-186 significantly promoted the proliferation of COPD MRC-5 cell

• 作者: Dong-Chuan Xu Chan-Yi He Qi Lin Yi-Peng Ding
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（11）
• 所属期刊栏目: Original article
• 页码范围: 14-18
• 页数: 5页
• 语种: 英文

摘要

Objective: To study the effect of cigarette extract (CSE) on the expression of exosomal miR-186 derived from 16HBE cells and the effects of 16HBE-derived exosomal miR-186 on the proliferation of MRC-5 cells. Methods: To collect the exosomal miR-186 in the supernatant of CSE-treated 16HBE cells for MRC-5 cell culture; the expression of exosomal miR-186 was detected by qPCR; the proliferation of MRC-5 cell was detected by CCK-8; dual-luciferase reporter gene experiment was used to detect the targeted regulation relationship between miR-186 and Bcl2L11; the expression of Bcl2L11 was detected by Western blot. Results: The morphology of 16HBE cells about 100 nm in diameter were observed by TEM; CSE treatment significantly promoted the expression of exosomal miR-186; CSE-induced exosomal miR-186 promoted the proliferation of MRC-5 cells; Bcl2L11 is a target gene of miR-186; miR-186 mimics significantly decreased Bcl2L11 expression.Conclusion: CSE-induced 16HBE-derived exosomal miR-186 promoted the proliferation of MRC-5 cells by targeting Bcl2L11 genes.