Analysis of 18S rDNA Sequence of the Pathogen of Melon Powdery Mildew from Lands Overlaid with Sands in Ningxia

• 作者: YANG Jing-ling LIU Jian-li
• 来源期刊: 植物病虫害研究：英文版
• 学科: 农学
• 关键词: 植物病虫害 预防 植物保护 植物检疫
• 年，卷（期）: 2012,（1）
• 页码范围: 14-16
• 页数: 3页
• 分类号: S4

摘要

[Objective] The paper was to measure and analyze pathogen of 18S rDNA sequence of the pathogen of melon powdery mildew from lands overlaid with sands. [Method]The melon powdery mildew was isolated from infected plants of 'Yujinxiang', a major melon variety cultivated in lands overlaid with sands in the middle arid area of Ningxia. Genome DNA was extracted from its conidia using Chelex-100 method. 18S rDNA sequence was amplified by PCR, which was analyzed by Blast after sequencing, and the phylogenetic tree was constructed. [Result] 18S rDNA sequence analysis showed that the pathogen of melon powdery mildew belonged to Podosphaera. [Conclusion] The study provided reference for biocontrol and disease-resistance breeding against melon powdery mildew.