Ozone action on Streptococcus mutans and Lactobacillus fermentum:A pilot study

• 作者: Anabela Paula Eunice Carrilho Joana Marques Manuel Ferreira Teresa Goncalves
• 来源期刊: 世界口腔医学杂志
• 学科: 医学
• 关键词: DENTAL CARIES Cariogenic AGENTS Lactobaccilus fermentum STREPTOCOCCUS MUTANS OZONE
• 年，卷（期）: 2013,（1）
• 页码范围: 18-23
• 页数: 6页
• 分类号: R

摘要

AIM:To study the effectiveness of ozone in the elimination of cariogenic bacteria,followed with fluoride supplements.METHODS:Sixty extracted teeth free of caries were used,and five groups were constituted.In GroupⅠ,the teeth were immersed in artificial saliva.In GroupⅡ,the teeth were inoculated with Streptococcus mutans(S.mutans)and immersed in artificial saliva.In GroupⅢthe teeth were inoculated with Lactobaccilus fermentum(L.fermentum)and immersed in artificial saliva.In GroupⅣthe teeth were inoculated with S.mutans and L.fermentum and immersed in artificial saliva and the teeth in GroupⅤwere inoculated with S.mutans and L.fermentum,and were subjected to the application of ozone and to the action of a fluoride mineralizing gel.DIAGNOdent was used to evaluate the caries of the teeth 3 wk after inoculation of bacteria and after that the teeth of GroupⅤwere subjected to the application of ozone during 60 s,by HealOzone.After the application of ozone,products of the remineralization kit supplied by the manufacturer were applied daily,during 30 d.At the end samples were collected for analysis and evaluation of bacterial activity by polymerase chain reaction.RESULTS:Regarding the value of caries,obtained via DIAGNOdent,in the initial measurement the groups are homogeneous(P=0.730).There was an increase in DIAGNOdent values,presenting statistical significant difference regarding the initial measurement in all groups(P?0.001),except in groupⅠ-only artificial saliva-which shows that the artificial carie model was effective.Comparing the initial and final measurements for each of the 60 teeth,it can be observed that in 9 teeth(15.0%)there was a decrease in values between the two measurements,one(1.7%)retained the same values in the two measurements and in the remaining 50 cases(83.3%)there was increase in values between the initial and final measurements.It should also be noted that in the teeth inoculated with S.mutans+L.fermentum,there was an increase of the values in 100%of cases,and in all groups except