Rapid DNA Extraction Methods for Direct-PCR Detec- tion Citrus Huanglongbing

• 作者: Wang Huatang Xue Peipei Yang Liu Zeng Xinnian
• 来源期刊: Plant Diseases and Pests(植物病虫害研究：英文版)
• 学科: 农学
• 关键词: DNA提取方法 快速提取方法 PCR检测 柑橘黄龙病 基因组DNA提取 定量分类 病原检测 制备工艺
• 年，卷（期）: 2015,（3）
• 页码范围: 1-4
• 页数: 4页
• 分类号: S792.14

摘要

[Objective] This study aimed to develop a simple and effective DNA extraction method for citrus Huanglongbing pathogen detection.[Method] From aspects of preparing procedure,prepare time and the quality of DNA,advantages and disadvantages of three sample preparation methods were compared,include two Direct-PCR extraction methods and one universal genomic DNA extraction kit method. In addition,PCR amplification effect on specific primers for 16S rDNA of " Candidatus Liberibacter asiaticus"( CLas) had also been evaluated.[Result] The results showed that RT-qPCR detected CLas by using DNA obtained from one of Direct-PCR extraction method as templates. Under improved Direct-PCR extraction method,16S rDNA of CLas could also be amplified by routine PCR.[Conclusion]A simple and effective DNA extraction method of citrus Huanglongbing pathogen have been established,which provides technical supports for preparation of large number of samples for detection of CLas.