Effects of Pulsed Electromagnetic Fields of Different Frequencies on the Gene Expression of RANK and NFATc1 in Rat Osteoclasts Co-Cultured with Osteoblasts

• 作者: Hui Huang Jian Chen Jianquan He Zhang Xin
• 来源期刊: 生物医学工程(英文)
• 学科: 医学
• 关键词: PEMFs Co-Cultured RANK MRNA NFATc1 MRNA
• 年，卷（期）: 2017,（1）
• 页码范围: 21-30
• 页数: 10页
• 分类号: R73

摘要

The study investigated the effects of pulsed electromagnetic fields (PEMFs) of different frequencies on the gene expression of receptor activator of nuclear factor kappa B (RANK) and Nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) in rat osteoblast and osteoclast co-cultured model. Osteoblast-like cells were isolated from calvariae of Newborn Sprague Dawley rats (SD rats), while osteoclast-like cells were obtained from femora and tibiae of five weeks old SD rats. After 1 days of co-culture, the cells were exposed to premarin (E2) and different frequencies of PEMFs (8 Hz and 16 Hz, respectively) for 3 days. The expression of RANK and NFATc1 mRNA was analysed with realtime quantitative polymerase chain reaction. The gene expression of RANK and NFATc1 in the E2, PEMF with 8 Hz and 16 Hz group was significantly lower than that in the control group respectively. The gene expression of NFATc1 in the PEMF with 8 Hz group was significantly lower than that in the control group and PEMF with 16 Hz group. The study indicates that PEMF with 8 Hz could regulate the gene expression of RANK and NFATc1 in co-cultured model.