可可毛色二孢菌侵染后的烟草酵母双杂交cDNA文库构建

• 作者: 刘梅 富春元 张玮 李兴红 燕继晔 邢启凯
• 来源期刊: 植物病理学报
• 学科: 农学
• 年，卷（期）: 2017,（5）
• 所属期刊栏目: 研究简报
• 页码范围: 716-720
• 页数: 5页
• 分类号: S432.44
• 语种: 中文
• DOI: 10.13926/j.cnki.apps.000030

摘要

The occurrence of grapevine canker caused by pathogens of fungal family Botryosphaeriaceae is increasing in most grapevine growing areas worldwide.However,the mechanism of Botryosphaeriaceae-grapevine interaction is still in its infancy.In order to identify Botryosphaeriaceae resistance-related genes and targets of fungal effectors in grapevine,a yeast two-hybrid cDNA library of tobacco infected by Lasiodiplodia theobromae CSS-01s was constructed by SMART cDNA synthesis technology.The results showed that the titer of cDNA library was 1.2×108 CFU · mL-1.Results of PCR amplification of 24 clones randomly selected from the cDNA library revealed that the length of inserted cDNA fragments ranged from 350 bp to 2 000 bp,with a recombination rate of 100％.In addition,the average insert size of the cDNA library was 750 bp,indicating that this library is of high quality and could be applied for subsequent research.

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	李兴红		63	550	13.0	20.0
2	燕继晔		17	113	7.0	10.0
3	张玮		16	112	6.0	10.0
4	刘梅		12	33	4.0	5.0
5	邢启凯		3	0	0.0	0.0
6	富春元		2	0	0.0	0.0