MicroRNA-155通过调节CXCR4/PI3 K/AKT途径影响滋养细胞的侵袭与迁移

• 作者: 刘慧 周洁 宋婉玉 张展 徐娜 李爱萍 王媛媛
• 来源期刊: 中国免疫学杂志
• 学科: 医学
• 关键词: miR-155 CXCR4 PI3K/AKT 滋养细胞 子痫前期
• 年，卷（期）: 2017,（1）
• 所属期刊栏目: 基础免疫学
• 页码范围: 41-46,51
• 页数: 7页
• 分类号: R392.12
• 字数: 4555字
• 语种: 中文
• DOI: 10.3969/j.issn.1000-484X.2017.01.008

摘要

Objective:To investigate the effect on the CXCR4/ PI3K/ AKT pathway after the transfection of miR-155 mimics and miR-155 inhibitor combined with the research on the ability of invasion and migration of human chorionic JEG-3 trophoblast cells. Methods: Chemically synthesized miR-155 mimics and miR-155 inhibitor were transfected into JEG-3 cells. The effect on the ability of invasion and migration were analyzed by Transwell migration assay and Wound healing assay. The expression of CXCR4 mRNA was detected by Real-time PCR. The expression of CXCR4 and p-AKT protein were detected by Western blot. Results: Transfection with miR-155 mimics significantly down-regulated the expression of CXCR4 as compared with the control group(P<0.05);JEG-3 cells transfected miR-155 mimics had lower levels of migration and invasion capacity than cells in the control group(P<0.05). However, transfection with miR-155 inhibitor significantly up-regulated the expression of CXCR4 as compared with the control group(P<0.05); JEG-3 cells transfected miR-155 inhibitor had higher levels of migration and invasion capacity than cells in the control group(P <0.05). Addition,the expression of p-AKT protein of JEG-3 cells was down-regulated after transfected miR-155 mimics,and the expression of p-AKT protein of JEG-3 cells was up-regulated after transfected miR-155 inhibitor. Conclusion: miR-155 may inhibits the invasion and migration of trophoblast cells by regulating CXCR4/ PI3K/ AKT pathway contributing to the development of preeclampsia.

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	张展		191	1024	15.0	21.0
2	刘慧		69	191	7.0	10.0
3	徐娜		15	85	4.0	9.0
4	李爱萍		13	36	4.0	5.0
5	周洁		19	89	5.0	9.0
6	王媛媛		17	51	5.0	6.0
7	宋婉玉		10	44	4.0	6.0