RNF219 interacts with CCR4-NOT in regulating stem cell differentiation

• 作者: Hao Du Chen Chen Yan Wang Yang Yang Zhuanzhuan Che Xiaoxu Liu Siyan Meng Chenghao Guo Manman Xu Haitong Fang Fengchao Wang Chengqi Lin Zhuojuan Luo
• 来源期刊: 分子细胞生物学报（英文版）
• 年，卷（期）: 2020,（11）
• 所属期刊栏目: Articles
• 页码范围: 894-905
• 页数: 12页
• 语种: 英文
• DOI: 10.1093/jmcb/mjaa061

摘要

Regulation of RNA stability plays a crucial role in gene expression control.Deadenylation is the initial rate-limiting step for the majority of RNA decay events.Here,we show that RING finger protein 219 (RNF219) interacts with the CCR4-NOT deadenylase complex.RNF219-CCR4-NOT exhibits deadenylation activity in vitro.RNA-seq analyses identify some of the 2-cell-specific genes and the neuronal genes significantly downregulated upon RNF219 knockdown,while upregulated after depletion of the CCR4-NOT subunit CNOT10 in mouse embryonic stem (ES) cells.RNF219 depletion leads to impaired neuronal lineage commitment during ES cell differentiation.Our study suggests that RNF219 is a novel interacting partner of CCR4-NOT and required for maintenance of ES cell pludpotency.