Effects of Lingguizhugan Decoction on α-SMA and collagen synthesis in rat myocardial fibroblasts induced by transforming growth factor-β1

• 作者: Rui-Rui Ge Xiang Wang Tong-Juan Tang Liang Wang Meng-Ting Zhai Meng-Yu Zuo Jian Chen Peng Zhou Jin-Ling Huang
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（10）
• 所属期刊栏目: Original article
• 页码范围: 19-24
• 页数: 6页
• 语种: 英文

摘要

Objective: To explore the protective effect of Linggui Zhugan Decoction (LGZGD) medicated serum on myocardial fibrosis induced by transforming growth factor-β1 (TGF-β1). Methods: Using enzyme digestion method, combined with differential adherence to isolate and culture Sprague-Dawley (SD) suckling mouse Cardiac fibroblasys (CFB) in vitro. Divided into: blank group, blank rat serum group, model group, and LGZGD medicated serum group (5％、10％、20％ ). Except for blank group and blank rat serum group, they were stimulated with 5 ng/ml TGF-β1 for 12 hours , and then then intervene with LGZGD medicated serum (5％、10％、20％ ) and continue to culture for 24 hours. Use immunofluorescence and Western blot (WB) to detect the expression of α-smooth muscle actin (α-SMA), Enzyme-linked immunosorbent assay (ELISA) and WB to detect type I collagen (Collagen I) , type I collagen (Collagen Ⅲ) and fibronectin (FN) expression. Results: Compared with the blank group, the expressions of Collagen I, Collagen Ⅲ, α-SMA and FN in the model group were significantly increased (P<0.01); Compared with the model group, the expressions of Collagen I and Collagen Ⅲ in each concentration group of the experiment were significantly reduced (P<0.01); the expression of α-SMA and FN were significantly reduced (P<0.01). Conclusions: LGZGD has an inhibitory effect on collagen synthesis and the expression of α-SMA and FN, indicating that the anti-fibrosis effect of LGZGD is related to it.