Optimization of the extraction process of total flavonoids from Trichosanthis Fructus by Box-Behnken response surface method combined with differential spectrophotometry

• 作者: Qian Hu Wan-Zhi Qian Chun-Cai Zou Hai-Yan Yan
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（11）
• 所属期刊栏目: Original article
• 页码范围: 26-31
• 页数: 6页
• 语种: 英文

摘要

Objective: To optimize the extraction process of total flavones in Trichosanthis Fructus (composed of Trichosanthis pericarpium and Trichosanthis semen in certain proportion). Methods: The effects of the mixture ratio of Trichosanthis pericarpium and Trichosanthis semen, ethanol concentration, ultrasonic extraction time and extraction temperature on the extraction rate of total flavonoids in Trichosanthis Fructus were investigated. The extraction process of total flavonoids in Trichosanthis Fructus was optimized by Box-Behnken response surface method combined with differential spectrophotometry. Results: The optimum extraction conditions of total flavonoids in Trichosanthis Fructus were as follows: The mixture ratio of Trichosanthis pericarpium and Trichosanthis semen was 4:6, the ethanol concentration was 70％, the ultrasonic extraction time was 60min and the extraction temperature was 40℃. Conclusion: Box-Behnken response surface method combined with differential spectrophotometry can optimize the extraction process of total flavonoids from Trichosanthis Fructus, which can provide reference for the extraction and application of total flavonoids in Trichosanthis Fructus.