Screening of Respiration Deficiency Mutants of Yeasts （Saccharomyces cerevisiae） Induced by Ion Irradiation and mtDNA Restriction Analysis

• 作者: Mao Shuhong Jin Genming Xie Hongmei Gu Ying Ma Qiufeng
• 来源期刊: 近代物理研究所和兰州重离子加速器实验室年报：英文版
• 学科: 生物学
• 关键词: 线粒体DNA 酿酒酵母 离子辐照 突变体 辐射诱发 缺陷 呼吸 筛选
• 年，卷（期）: 2004,（1）
• 页码范围: 102
• 页数: 1页
• 分类号: Q959.468

摘要

<正>A screen of respiration deficiency mutants of Saccharomyces cerevisiae induced by 5.19 MeV/u 22Ne5+ ion irradiation is studied. Some respiration deficiency mutants, which present white colony phenotype on condition of selective culture of TTC medium, were obtained. A new and simplified method based on mtDNA restriction analysis is used, and mitochondrial restriction fragments are easily observed by agarose gel electrophoresis. In this way, we found that there are many obvious differences in RFLP of mtDNA between the wild yeasts and the respiration deficiency mutants. As shown in Fig.1, compared with the wild yeasts, the respiration deficiency mutants have large deletions and new restriction patterns in mtDNA.