Engineering stem cell niches in bioreactors

• 作者: Meimei Liu Ning Liu Ru Zang Shang-Tian Yang Yan Li
• 来源期刊: 世界干细胞杂志：英文版（电子版）
• 学科: 医学
• 关键词: STEM cell ENGINEERING BIOREACTOR DIFFERENTIATION MICROENVIRONMENT MICROFLUIDICS
• 年，卷（期）: 2013,（4）
• 页码范围: 124-135
• 页数: 12页
• 分类号: R329

摘要

Stem cells, including embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells and amniotic fluid stem cells have the potential to be expanded and differentiated into various cell types in the body.Efficient differentiation of stem cells with the desired tissue-specific function is critical for stem cell-based cell therapy, tissue engineering, drug discovery and disease modeling. Bioreactors provide a great platform to regulate the stem cell microenvironment, known as 'niches',to impact stem cell fate decision. The niche factors include the regulatory factors such as oxygen, extracellular matrix(synthetic and decellularized), paracrine/autocrine signaling and physical forces(i.e., mechanical force, electrical force and flow shear). The use of novel bioreactors with precise control and recapitulation of niche factors through modulating reactor operation parameters can enable efficient stem cell expansion and differentiation. Recently, the development of microfluidic devices and microbioreactors also provides powerful tools to manipulate the stem cell microenvironment by adjusting flow rate and cytokine gradients. In general,bioreactor engineering can be used to better modulate stem cell niches critical for stem cell expansion, differentiation and applications as novel cell-based biomedicines. This paper reviews important factors that can be more precisely controlled in bioreactors and their effects on stem cell engineering.