Cloning and Expression of One Anthocyanin-Related R2R3-MYB Gene in <i>Rosa rugosa</i>

• 作者: Lanyong Zhao Mingyuan Zhao Xiaoming Sui Xu Han Yang Wang Zongda Xu
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: ROSA RUGOSA ANTHOCYANIN R2R3-MYB GENE Expression
• 年，卷（期）: 2018,（10）
• 页码范围: 2020-2032
• 页数: 13页
• 分类号: R73

摘要

Based on the transcriptome of Rosa rugosa, one anthocyanin-promoting R2R3-MYB gene, RrMYB10.1 (Accession Nos:MH717244), was cloned from the petals of Rosa rugosa ‘Zizhi’. Sequence analysis results showed that RrMYB10.1 had a full length opening reading frame of 747bp, encoding 249 amino acids. Sequence analysis revealed that RrMYB10.1 contained the conserved R2R3-MYB domain, two atypical anthocyanin-promoting motifs and a conserved amino acid signature for the interaction with bHLH protein. The results of phylogenic tree revealed that RrMYB10.1 showed high homology with other anthocyanin-promoting proteins in Rosacea, and sharing the highest identity (98.39%) with RhMYB10. RT-PCR results showed that RrMYB10.1 was mainly expressed in petals among various tissues and expressed significantly higher in petals in bud stage than in opening period. To sum up, these results showed that RrMYN10.1 may play a key role in regulating anthocyanin concentration, thus providing a certain foundation on regulating flower color formation in Rosa rugosa.