Factors involved in the success of Castanea henryi stem cuttings in different cutting mediums and cutting selection periods

• 作者: Wangzun Chen Libing He Shiyi Tian Joseph Masabni Huan Xiong Feng Zou Deyi Yuan
• 来源期刊: 林业研究（英文版）
• 年，卷（期）: 2021,（4）
• 所属期刊栏目: ORIGINAL PAPERS
• 页码范围: 1627-1639
• 页数: 13页
• 语种: 英文

摘要

Chinquapin ( Castanea henryi ) is a dual-purpose tree species in China valued for as a source of timber and starch. We investigated the effect of four cutting mediums (pure vermiculite; peat:river sand at 3:1 v/v; peat:krasnozem at 1:1 v/v; and pure krasnozem) and three stem cutting periods (March, May, and July) on rooting performance of C. henryi cuttings. Different cutting periods and cutting mediums greatly influenced the rooting rate of C. henryi, ranging from 3.35 to 77.31％. Principal component anal-ysis indicated that the best combination of cutting period and cutting medium was semi-hardwood cuttings (May cuttings) + krasnozem. Histological evidence indicated that adventitious root initials were present by week 5-6, and that the site of root primordia initiation was observed in the vascular cambium. Stem anatomical structures observed at different periods indicated that a xylem/radius ratio of 29.90-37.42％ and a fractured phloem fiber ring are indica-tive of rooting success. The relational model between root-ing index and medium properties indicated that nutrient con-tent and porosity significantly influenced callus production. However, pH strongly affected C. henryi root formation, with the Pearson correlation coefficients for May and July cuttings of ? 0.856 and ? 0.947, respectively. Our protocol is helpful to achieve mass clone propagation of improved C. henryi genotypes, thus overcoming a common hurdle in chinquapin breeding programs.