Development of high-oil maize haploid inducer with a novel phenotyping strategy

• 作者: Chenxu Liu Jinlong Li Ming Chen Wei Li Yu Zhong Xin Dong Xiaowei Xu Chen Chen Xiaolong Tian Shaojiang Chen
• 来源期刊: 作物学报（英文版）
• 年，卷（期）: 2022,（2）
• 所属期刊栏目: Research Papers
• 页码范围: 524-531
• 页数: 8页
• 语种: 英文

摘要

Doubled haploid(DH)technology is important in modern maize breeding.Haploid inducers determine the efficiency of both haploid induction and identification.It has taken decades to improve the efficiency,haploid induction rate(HIR),from the～2％of the ancestor haploid inducer,stock6,to the～10％of modern haploid inducers.Improvement of kernel oil content(KOC)would further enhance haploid identification efficiency.Using molecular marker-assisted selection,in combine with the number of haploids per ear as phenotypic criterion,we developed a new high-oil haploid inducer line,CHOI4,with a mean HIR of 15.8％and mean KOC of 11％.High KOC of CHOI4 can achieve a mean accuracy greater than 90％in identification of haploids of different backgrounds,with reduced false discovery rates and false negative rates in com-parison with the previous high-oil haploid inducer line,CHOI3.Comparison of phenotypic selection strategies suggested that the number of haploids per ear can be used as a phenotyping criterion during haploid inducer line development.CHOI4 could further increase the efficiency of large-scale DH breeding programs with lower cost.