Recombinant Lactobacillus plantarum NC8 strain expressing porcine rotavirus VP7 induces specific antibodies in BALB/c mice

• 作者: Seria Masole Shonyela Chunwei Shi Wentao Yang Xin Cao Guilian Yang Chunfeng Wang
• 来源期刊: 生物化学与生物物理学报（英文版）
• 年，卷（期）: 2021,（6）
• 所属期刊栏目: Original Articles
• 页码范围: 707-718
• 页数: 12页
• 语种: 英文
• DOI: 10.1093/abbs/gmab050

摘要

The major etiologic agent that causes acute gastroenteritis worldwide in young animals and chil-dren is Group A rotavirus.Currently,commercially available vaccines do not often prevent porcine rotavirus(PRV)infection.In this study,we evaluated the efficacy of oral recombinant Lactobacil-lus vaccine against PRV in a mouse model.Lactobacillus plantarum NC8 was used as the host strain,and bacterial vectors were constructed,because the NC8 isolated has shown the capability to survive gastric transit and to colonize the intestinal tract of humans and other mammals.To explore the immunological mechanisms,lactic acid bacterial vectors were used to express VP7 antigen from PRV.We constructed an L.plantarum strain with surface-displayed VP7,named NC8-pSIP409-pgsA-VP7-DCpep.The expressed recombinant protein had a molecular weight of～37 kDa.The strain was used to immunize BALB/c mice to evaluate their immunomodulatory characteris-tics.Mice were orally immunized with recombinant L.plantarum NC8-pSIP409-pgsA-VP7-DCpep at a dose of 2 x 109 colony forming units/200 μl.The results showed that NC8-pSIP409-pgsA-VP7-DCpep significantly stimulated the differentiation of dendritic cells(DCs)in Peyer's patches(PPs)and increased the serum levels of IL-4 and IFN-y,as measured by enzyme-linked immunosorbent assay in mice treated with NC8-pSIP409-pgsA-VP7-DCpep.Compared to the empty vector group,NC8-pSIP409-pgsA-VP7-DCpep significantly increased the production of B220+B cells in mesen-teric lymph nodes(MLNs)and PPs and also increased the titer levels of the VP7-specific antibodies,including IgG and slgA.The administration of NC8-pSIP409-pgsA-VP7-DCpep mediated relatively broad cellular responses.This study reveals that clear alternatives exist for PRV control strategies and provides information on PRV infection.