Application of CRISPR-Cas9 technology in the screening of tumor resistance genes: A review

• 作者: Jie Zhang Tan Yang Yong-Gang Lu
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（11）
• 所属期刊栏目: Review and lectures
• 页码范围: 53-55
• 页数: 3页
• 语种: 英文

摘要

Drug resistance is a major problem faced by tumor cell-targeted drugs. Currently, functional gene screening is the most common strategy for screening drug resistance genes. In recent years, Crispr-cas9 gene editing technology has been widely used in the functional studies of tumor-related genes due to their characteristics of accuracy, simplicity and efficiency. The principle of CRISPR-Cas9 Library Screening Technology and its application in functional Gene Screening are reviewed. At the same time, the application prospect of the Crispr-Cas9 technology is forecasted.