Effects of Qishen Yiqi dripping pills-containing serum on KATP channel opening and PI3K/AKT signaling pathway in hypoxic/reoxygenated H9C2 cardiocytes

• 作者: Gui-Xin He Yu-Fei Feng Wei-Bin Qin Lin Lin Meng-Xian Hu Guo-Kun Zheng Li-Yan Yu Zi-Yong Jia Juan Wei Qi Wen
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2022,（6）
• 所属期刊栏目: Original article
• 页码范围: 12-18
• 页数: 7页
• 语种: 英文

摘要

Objective: To investigate the effects of Qishen Yiqi dropping pills serum on KATP channel opening and PI3K/AKT signaling pathway of hypoxic/reoxygenated H9C2 cardiocytes. Methods: H9C2 cardiocytes cultured in vitro were randomly divided into five groups, A: H9C2 cell group B: H9C2 cells +H2O2 model group C:H9C2 cells +H2O2 model + Qishen Yiqi group D:H9C2 cells +H2O2 model + Qishen Yiqi +wort group E:H9C2 cells +H2O2 model + Qishenyiqi +5-HD group, the drug intervention is according to the corresponding conditions. CCK-8 method was used to detect the cell activity of each group; Western blot was used to detect the expression of AKT and P-Akt proteins in myocardial cells in each group. The current was recorded by the standard patch clamp whole cell recording method, and the current was collected and analyzed by Pclamp6.0 software. Results: CCK-8 test results showed that compared with group A, the activity of myocardial cells in group B was significantly decreased, and the difference was statistically significant (P<0.01); compared with group B, the difference in group C was statistically significant (P<0.01); compared with C, cardiomyocyte activity in D and E group were significantly decreased, and the difference was statistically significant (P<0.05); WB results showed that compared with A, p-Akt protein expression in B, C, D and E groups were significantly decreased, and the difference was statistically significant (P< 0.01); compared with group B, p-Akt protein expression in C, D and E group were significantly increased, and the difference was statistically significant (P< 0.01),but there was no significant difference in AKT expression among groups (P>0.05); The results of whole cell patch clamp experiment showed that the outward current of B was significantly increased compared with that of A, and the difference between groups was statistically significant (P<0.01); compared with group B, cardiomyocytes in group C further increased the outward current, and the difference between groups was statistically significant (P<0.01); compared with C, the current of D and E group were significantly decreased, with statistical significance between groups (P<0.01). Conclusion: QishenYiqi dropping pills can protect cardiomyocytes by activating p-Akt protein expression and KATP channel opening in H9C2 cardiomyocytes.