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摘要:
The skin contains various populaions of stem cells, but its characterization has been hampered by lack of markers and unclear location. The hair follicle has a niche for stem cells called a “bulge” which acts as a reservoir of multipotent stem cells. In the study reported here, an immunohistochemical and immunofluorescence analysis was performed on mouse and human tissues in order to determine the possible presence of stem cells of hair follicle through cytokeratin 15 (CK15), CD34, and CD200 markers identified as crucial to the stem cells and to identify the bulge region. Mouse (n = 7) and human (n = 7) skin samples were used. The expression of proteins was determined by the indirect immunoperoxidase technique and a secondary antibody bound to a fluorochrome. The specificity of staining was evaluated by negative controls. The results revealed that the stem cells associated with CD34 and CD200 antibodies were differentially expressed in the interfollicular epidermis, sebaceous glands, and bulge region, indicating that, in mice, CD34 and, in humans, CD200 are more specific than CK15 in detecting bulge cells. It also suggests that CD34 is specific for mouse bulge cells, while CD200 might have specificity for progenitor cells and partially differentiated cells in humans.
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篇名 Identification of Bulge Stem Cells in Mouse and Human Hair Follicles
来源期刊 显微镜研究(英文) 学科 医学
关键词 STEM Cells HAIR Follicle BULGE CD34 CD200
年,卷(期) 2018,(3) 所属期刊栏目
研究方向 页码范围 19-29
页数 11页 分类号 R73
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研究主题发展历程
节点文献
STEM
Cells
HAIR
Follicle
BULGE
CD34
CD200
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期刊影响力
显微镜研究(英文)
季刊
2329-3306
武汉市江夏区汤逊湖北路38号光谷总部空间
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34
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0
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