Ex vivo expansion of hematopoietic stem and progenitor cells:Recent advances

• 作者: Fangming Xiu Katsuhiro Kita Marc G Jeschke
• 来源期刊: 世界血液学杂志
• 学科: 医学
• 关键词: HEMATOPOIETIC STEM CELL Ex vivo expansion SERUM-FREE culture CELL surface MARKERS ENRICHMENT STEM CELL isolation
• 年，卷（期）: sjxyxzz,（2）
• 页码范围: 18-28
• 页数: 11页
• 分类号: R

摘要

Hematopoietic stem cells(HSCs)have become the most extensively studied stem cells and HSC-based cellular therapy is promising for hematopoietic cancers and hereditary blood disorders.Successful treatment of patients with HSC cells depends on sufficient number of highly purified HSCs and progenitor cells.However,stem cells are a very rare population no matter where they come from.Thus,ex vivo amplification of these HSCs is essential.The heavy demands from more and more patients for HSCs also require industrial-scale expansion of HSCs with lower production cost and higher efficiency.Two main ways to reach that goal:(1)to find clinically applicable,simple and efficient methods(or reagents)to enrich HSCs;(2)to find new developmental regulators and chemical compounds in order to replace the currently used cytokine cocktails for HSCs amplification.In this Editorial review,we would like to introduce the current status of ex vivo expansion of HSCs,particularly focusing on enrichment and culture supplements.