螺旋藻激酶的体外抗氧化作用研究Δ

• 作者: 凌家杰 庞辉 李映新 王慧杰 纪舒妤 黄为然 黄媛恒 黎钦蓉
• 来源期刊: 中国药房
• 学科: 医学
• 关键词: 螺旋藻激酶 体外 抗氧化 自由基
• 年，卷（期）: 2016,（16）
• 所属期刊栏目: 实验研究
• 页码范围: 2184-2186
• 页数: 3页
• 分类号: R285.5
• 字数: 3571字
• 语种: 中文
• DOI: 10.6039/j.issn.1001-0408.2016.16.07

摘要

ABSTRACT OBJECTIVE：To study the anti-oxidative effects of spirulina kinase （SPK） in vitro. METHODS：The methods of phenanthroine-Fe2+ oxidation method，DPPH and auto-oxidation of pyrogallol were used to measure the effects of different concen-trations of SPK on scavenging hydroxyl （OH－） free radical，DPPH free radical and superoxide anion （O2－） free radical；IC50 of SPK was calculated. Prussian blue reaction was used to determine total reducing ability（by absorbance）of different concentrations of SPK to Fe3+. Vitamin C（VC）was used as positive control in above trials. RESULTS：SPK could eliminate the OH－free radical， DPPH free radical and O2－ free radical in concentration-dependant manner，and the maximum elimination rate of SPK to OH－ free radical and DPPH free radical were 86.82％ and 78.98％（IC50 were 54.31，0.636 g/L），which were higher than VC （64.77％，73.49％）. The maximum elimination rate of SPK to O2－ free radical was 78.31％（IC50 was 3.918 g/L），which was lower than VC （94.14％）. In reducing ability test，SPK improved absorbance in reducing ability test system，and maximum absorbance was simi-lar to VC in concentration-dependant manner. CONCLUSIONS：SPK has obvious anti-oxidant activities in vitro.

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	李映新	广西医科大学药学院	33	79	5.0	7.0
2	庞辉	广西医科大学基础医学院	43	230	9.0	12.0
3	黄媛恒	广西医科大学基础医学院	23	83	5.0	8.0
4	王慧杰	广西医科大学基础医学院	12	55	4.0	7.0
5	黎钦蓉	广西医科大学全科医学院	3	5	1.0	2.0
6	凌家杰	广西医科大学基础医学院	2	2	1.0	1.0
7	纪舒妤	广西医科大学全科医学院	2	13	1.0	2.0
8	黄为然	广西医科大学全科医学院	2	13	1.0	2.0