Taqman PCR-based Methods for Rapid Detection o＇ Salmonella in Pet Food

• 作者: CUI He JIA Jun-tao JIANG Ying-hui LI Zheng-yi MA Yun ZENG Jing
• 来源期刊: 中国食品科学：英文版
• 学科: 工学
• 关键词: 荧光定量PCR检测 沙门氏菌感染 宠物食品 PCR方法 实时PCR 测点 TAQMAN 核苷酸序列
• 年，卷（期）: 2012,（3）
• 页码范围: 32-34
• 页数: 3页
• 分类号: TS207.3

摘要

[Objective] To establish a Taqman real-time PCR for detection of Salmonella in pet food. [Method] A pair of primers and a probe were designed based on published nucleotide sequence of invA gene encoding the invasion protein of Salmonella enterica. [Result] The assay detects Salmonella specifically. The detection limit of the real-time PCR was 17 CFU/test (25 μL/test) for the positive strain. This method was effective to detect artificially contaminated pet food. [Conclusion] The results showed that Taqman PCR assay was rapid and accurate for detection of Salmonella from infected pet food.