Pluripotent stem cell-derived neural stem cells: From basic research to applications

• 作者: Masahiro Otsu Takashi Nakayama Nobuo Inoue
• 来源期刊: 世界干细胞杂志：英文版（电子版）
• 学科: 医学
• 关键词: PLURIPOTENT STEM CELLS EMBRYONIC STEM CELLS NEURAL
• 年，卷（期）: 2014,（5）
• 页码范围: 651-657
• 页数: 7页
• 分类号: R329.2

摘要

Basic research on pluripotent stem cells is designed to enhance understanding of embryogenesis, whereas applied research is designed to develop novel therapies and prevent diseases. Attainment of these goals has been enhanced by the establishment of embryonic stem cell lines, the technological development of genomic reprogramming to generate induced-pluripotent stem cells, and improvements in in vitro techniques to manipulate stem cells. This review summarizes the techniques required to generate neural cells from pluripotent stem cells. In particular, this review describes current research applications of a simple neural differentiation method, the neural stem sphere method, which we developed.