In the present work,zinc oxide(ZnO)and silver(Ag)doped ZnO nanostructures are synthesized using a hydrotherm-al method.Structural quality of the products is attested using X-ray diffraction,which confirms the hexagonal wurtzite struc-ture of pure ZnO and Ag-doped ZnO nanostructures.XRD further confirms the crystallite orientation along the c-axis,(101)plane.The field emission scanning electron microscope study reveals the change in shape of the synthesized ZnO particles from hexagonal nanoparticles to needle-shaped nanostructures for 3 wt%Ag-doped ZnO.The optical band gaps and lattice strain of nanostructures is increased significantly with the increase of doping concentration of Ag in ZnO nanostructure.The anti-microbial activity of synthesized nanostructures has been evaluated against the gram-positive human pathogenic bacteria,Sta-phylococcus aureus via an agarose gel diffusion test.The maximum value of zone of inhibition(22 mm)is achieved for 3 wt%Ag-doped ZnO nanostructure and it clearly demonstrates the remarkable antibacterial activity.